Varga-Biro-Pejtsik: The shape of excitment

2018 Nikon-KOKI image contest results

Auro Science News

Images

In the image category the first three winners honored with camera or binoculars sponsored by Nikon CEE and Auroscience Kft.. There were 11 candidates and 41 images.

10. László Bíró: Hydra Invasions

László Bíró: Hydra invasion

Description: Fixed mouse visual cortical brain section, 30um
green: Perineuronal nets labeled with Lectin from Wisteria floribunda agglutinin visualized by Alexa 488–conjugated secondary antibody
blue: parvalbumin labeled with anti-parvalbumin visualized by Alexa 647–conjugated secondary antibody
Microscopy method: Confocal, 20x

9. Éva Krizsán: The Surface of the Sun

Krizsan Eva: The Surface of the Sun

Description: vGAT/IRES-cre//Gt(ROSA)26Sor_CAG/LSL_ZsGreen1 (TmTm) : Bl6Fx transgenic mouse brain fixed with 4% PFA, coronal section, 50um
medial globus pallidus, cerebral peduncle (basal part)
ZsGreen1 transgenic mouse
Microscopy method: A1R confocal, 20x

8. Tamás Wilheim: I got you

Wilheim Tamás: I got you

Description: Fixated rat brain section after 1h ischaemia, and 1h re-perfusion.
Staining:
green: ezrin primary with FITC secondary
red: IBA1 primary, CY3 secondary.
Microscopy method: A1R confocal, 60x oil, 3D reconstructed, with Amira

7. Erzsébet Gregori: Flames

Gregori Erzsebet: Flames

Description: 4% PFA fixed PVeGFP mouse; horizontal brain section; striatum (50 μm) Labeling:
Yellow: goat GFP labeled with Alexa 488 Donkey Anti-Goat
Red: rabbit Synaptotagmin2 lebeled with Cy3 Donkey Anti-Rabbit
Microscopy method: Nikon C2 confocal microscope, 60x magnification

6. Balázs Újfalussy: Bonsai Trees

Újfalussy Balázs: Bonsai Trees

Description: Manganese dendrites (mineral forms) on a piece of stone
Labeling: –
Microscopy method: Canon G15 digital camera

5. Barbara Orsolits: Carnival

Orsolits Barbara: Carnival

Description: Fixed astroglial culture
Blue: CytC positive mitochondria, Red: astrocytes labeled with anti-GFAP, Yellow: nuclei labeled with hoechst
Microscopy method: Confocal, 60x

4. Benjamin Barti: Unmoved movers

Barti Benjamin: Unmoved movers

Description: Fixed mouse cerebellar brain section, 300 um

Labeling:
Faint-orange: DAPI; Red: biocytin filled Purkinje Cells visualized by streptavidin 488; Blue: GFAP labeled with anti-GFAP, visualized by CF568 secondary antibody.

Microscopy method: Confocal, 20x

3. Gyula Balla: The long arm of coincidence

Balla Gyula: The long arm of coincidence

Description: fixed mouse brain section (Globus pallidus), 50 um
Labelings: blue – DAPI, visualized by DyLight 405, green: labelled with anti Gpr158, visualized by Alexa594, far-red: CB1 – labelled with anti-CB1, visualized with Alexa647
Microscopy method: confocal, 20x
Equipment:   Nikon A1R – confocal imaging
Software: NIS Elelents AR

2. Judit Glavinics: Meteor Shower

Glavinics Judit: Meteor Shower

Description: fixed mouse hippocampal brain section, 20um
Labeling: CB1 receptor labeled with anti-CB1 visualized by Alexa 488Ėconjugated secondary antibody, NECAB2 labeled with anti-NECAB2 visualized by Alexa 561Ėconjugated secondary antibody, DAPI
Microscopy method: confocal, 20x

1.  Varga-Biro-Pejtsik: The shape of excitment

Varga-Biro-Pejtsik: The shape of excitment

Description: Cell activation pattern in response to novelty stress, fixed zebrafish whole-brain sample
white: DAPI
“fire”: pErK labeled with anti-pErK visualised by alexa 488-conjugated s.a.
Microscopy method: confocal, 20x large image

Video

In the Video category the winner honored with digital camera sponsored by Supertech Kft.

1.  Prokop Susanne: Invasion

Author list:  Susanne Prokop, László Barna, István Katona Institure of Experimental Medicine
Description: Transiently transfected HEK293 cells, live
Labeling: GFP-tagged CB1 cannabinoid receptor, fluorescent protein fused with CB1
Microscopy method: live cell confocal microscopy, A1R, 60X water immersion objective