Dual-channel 3D-STORM image of an axon terminal decorated with CB1 cannabinoid receptors

Anatomical evidence for protein translation in nerve endings with STORM

Auro Science News

Electron microscopic studies failed to detect the translation machinery in mature mammalian axon terminals for decades. In this study, exploiting the single-molecule sensitivity of N-STORM, scientists from KOKI and the Albert Einstein College of Medicine demonstrated that ribosomal RNA and protein are present in inhibitory axon terminals of the hippocampus, and that local presynaptic protein synthesis is required for the long-term plasticity at these synapses.

Image: Dual-channel 3D-STORM image of an axon terminal decorated with CB1 cannabinoid receptors (blue), containing clustered labeling against the 5.8S ribosomal RNA (green).

Full article: Younts et al., Neuron (2016) 92:479-492.

DOI: 10.1016/j.neuron.2016.09.040