03_Galaxy_after_10_pm

Galaxy after 10 pm

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Dani Schlingloff After electrophysiological recording, the biocityn filled hippocampal interneuron (PV+ Basket Cell, in mouse brain slice) was visualised by using two different streptavidin conjugated Alexa-dyes together by accident (Streptavidin-Alexa488 and Straptavidin-Alexa594). The photo was taken with A1R (with 60x magnification). In the deeper layers, the Alexa-594 had weaker labeling, which caused a beautiful color gradient from red to green …





02_Welcome_to_our_galaxy

Welcome to our galaxy

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Dani Schlingloff After electrophysiological recording, the biocityn filled hippocampal interneuron (PV+ Basket Cell, in mouse brain slice) was visualised by using two different streptavidin conjugated Alexa-dyes together by accident (Streptavidin-Alexa488 and Straptavidin-Alexa594). The photo was taken with A1R (with 60x magnification). In the deeper layers, the Alexa-594 had weaker labeling, which caused a beautiful color gradient from red to green …





stocking

Stocking

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Rita Karlocai Glial fibrillary acidic protein (GFAP in green) staining with nucleus staining in blue (DAPI) and type 2 chloride channel-staining (red). Staining was carried out in the mouse hippocampus.



twins2

Twins 2

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Rita Karlocai Biocytin-filled CA1 pyramidal cell (red) labeled in a genetically modified mouse, where parvalbumin-positive cells express chanellrhodopsin and YFP in their membranes (green cell). Note that the YFP signal is so strong, that no further enhancement is necessary for microscopic visualization. Artificially modified colours.



twins

Twins

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Rita Karlocai Biocytin-filled CA1 pyramidal cell (red) labeled in a genetically modified mouse, where parvalbumin-positive cells express chanellrhodopsin and YFP in their membranes (green cell). Note that the YFP signal is so strong, that no further enhancement is necessary for microscopic visualization.



cell_on_ice

Cell on ice

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Rita Karlocai Biocytin labeled parvalbumin-positive cell (blue) filled in mouse hippocampal region CA3 immunostained for SATB (orange nucleus) to differentiate basket cells from axo-axonic cells. Colours were artificially modified.



01_Spring_basket

Spring basket

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Dani Schlingloff After electrophysiological recording, the biocityn filled hippocampal interneuron (PV+ Basket Cell, in mouse brain slice) was visualised by using two different streptavidin conjugated Alexa-dyes together by accident (Streptavidin-Alexa488 and Straptavidin-Alexa594). The photo was taken with A1R (with 60x magnification). In the deeper layers, the Alexa-594 had weaker labeling, which caused a beautiful color gradient from red to green …





06_scream

Scream

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Gyula Balla Mouse brain section (50um thick) labelled against Vesicular GABA transporter (VGAT) (Alexa488) and Cannabinoid Receptor 1 (CB1) (Alexa594) +DAPI staining. Image taken from hippocampal CA3 pyramidale.
CA3 radiatum, Orange coloured regions labels the colocalization of CB1 and VGAT
Magnification: 60x, 0,06 nm/px
Equipment:  Nikon A1R – confocal imaging
Software: Nikon Elelents AR



Atlas_original

Atlas

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Viktor Plattner In vivio recorded neuron in the intralaminar thalamus filled with neurobiotin (blue) surrounded by glycinergic fibers (yellow) in a glycine transporter 2 – eGFP transgenic mouse.



04_eye of the chameleon

Eye of the chameleon

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Gyula Balla Mouse brain section (50um thick) labelled against Vesicular GABA transporter (VGAT) (Alexa488) and Cannabinoid Receptor 1 (CB1) (Alexa594) +DAPI staining.
Substantia nigra and thalamic parts,  Orange coloured regions labels the extensive colocalization of CB1 and VGAT
Magnification: 20x
Equipment:  Nikon A1R – confocal imaging
Software: Nikon Elelents AR