Galaxy after 10 pm

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Dani Schlingloff After electrophysiological recording, the biocityn filled hippocampal interneuron (PV+ Basket Cell, in mouse brain slice) was visualised by using two different streptavidin conjugated Alexa-dyes together by accident (Streptavidin-Alexa488 and Straptavidin-Alexa594). The photo was taken with A1R (with 60x magnification). In the deeper layers, the Alexa-594 had weaker labeling, which caused a beautiful color gradient from red to green …

Welcome to our galaxy

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Dani Schlingloff After electrophysiological recording, the biocityn filled hippocampal interneuron (PV+ Basket Cell, in mouse brain slice) was visualised by using two different streptavidin conjugated Alexa-dyes together by accident (Streptavidin-Alexa488 and Straptavidin-Alexa594). The photo was taken with A1R (with 60x magnification). In the deeper layers, the Alexa-594 had weaker labeling, which caused a beautiful color gradient from red to green …

Stocking

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Rita Karlocai Glial fibrillary acidic protein (GFAP in green) staining with nucleus staining in blue (DAPI) and type 2 chloride channel-staining (red). Staining was carried out in the mouse hippocampus.

Twins 2

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Rita Karlocai Biocytin-filled CA1 pyramidal cell (red) labeled in a genetically modified mouse, where parvalbumin-positive cells express chanellrhodopsin and YFP in their membranes (green cell). Note that the YFP signal is so strong, that no further enhancement is necessary for microscopic visualization. Artificially modified colours.

Twins

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Rita Karlocai Biocytin-filled CA1 pyramidal cell (red) labeled in a genetically modified mouse, where parvalbumin-positive cells express chanellrhodopsin and YFP in their membranes (green cell). Note that the YFP signal is so strong, that no further enhancement is necessary for microscopic visualization.

Cell on ice

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Rita Karlocai Biocytin labeled parvalbumin-positive cell (blue) filled in mouse hippocampal region CA3 immunostained for SATB (orange nucleus) to differentiate basket cells from axo-axonic cells. Colours were artificially modified.

Spring basket

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Dani Schlingloff After electrophysiological recording, the biocityn filled hippocampal interneuron (PV+ Basket Cell, in mouse brain slice) was visualised by using two different streptavidin conjugated Alexa-dyes together by accident (Streptavidin-Alexa488 and Straptavidin-Alexa594). The photo was taken with A1R (with 60x magnification). In the deeper layers, the Alexa-594 had weaker labeling, which caused a beautiful color gradient from red to green …

Scream

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Gyula Balla Mouse brain section (50um thick) labelled against Vesicular GABA transporter (VGAT) (Alexa488) and Cannabinoid Receptor 1 (CB1) (Alexa594) +DAPI staining. Image taken from hippocampal CA3 pyramidale.
CA3 radiatum, Orange coloured regions labels the colocalization of CB1 and VGAT
Magnification: 60x, 0,06 nm/px
Equipment:  Nikon A1R – confocal imaging
Software: Nikon Elelents AR

Atlas

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Viktor Plattner In vivio recorded neuron in the intralaminar thalamus filled with neurobiotin (blue) surrounded by glycinergic fibers (yellow) in a glycine transporter 2 – eGFP transgenic mouse.

Eye of the chameleon

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Gyula Balla Mouse brain section (50um thick) labelled against Vesicular GABA transporter (VGAT) (Alexa488) and Cannabinoid Receptor 1 (CB1) (Alexa594) +DAPI staining.
Substantia nigra and thalamic parts,  Orange coloured regions labels the extensive colocalization of CB1 and VGAT
Magnification: 20x
Equipment:  Nikon A1R – confocal imaging
Software: Nikon Elelents AR