Hippocampal Montage

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Chris Henstridge These are primary hippocampal neurons transfected with the orphan receptor GPR35. The N-terminus of the receptor is tagged with SEP, a pH variant of GFP which only fluoresces under neutral pH conditions. Therefore, when expressed on the cell surface, the N-terminus of the receptor projects outwards into the neutral medium and can fluoresce following excitation. This cell was …

HEK Cell 3D

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Chris Henstridge This is a HEK293 cell transfected with the orphan receptor GPR35. The N-terminus of the receptor is tagged with SEP, a pH variant of GFP which only fluoresces under neutral pH conditions. Therefore, when expressed on the cell surface, the N-terminus of the receptor projects outwards into the neutral medium and can fluoresce following excitation. This cell was …

Surrounded 2

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Cserep Posfai Same area with an added staining of vGluT3 (green). Here you can also see the baskets formed by vGluT3 positive interneuron-terminals in stratum pyramidale. Photo made with Nikon A1R confocal microscope (60x, oil).