STORM image

New paper in Acta Neuropathologica by KOKI researchers, using correlated STORM super-resolution imaging: “Microglia control the spread of neurotropic virus infection via P2Y12 signalling and recruit monocytes through P2Y12-independent mechanisms”

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“Using a well-established model of alphaherpesvirus infection that reaches the brain exclusively via retrograde transsynaptic spread from the periphery, and in vivo two-photon imaging combined with high resolution microscopy, we show that microglia are recruited to and isolate infected neurons within hours. Selective elimination of microglia results in a marked increase in the spread of infection and egress of viral …

Co-transmission of acetylcholine and GABA regulates hippocampal states” published in Nature Communications by KOKI researchers, localised vesicle pools using STORM super-resolution imaging. Congratulation!

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Challenging a decades-old model of non-synaptic transmission of acetylcholine, we show that all hippocampal cholinergic terminals establish GABAergic synapses. We demonstrate that these synapses co-transmit GABA and acetylcholine via different vesicles, whose release is triggered by distinct calcium channels. This co-transmission evokes composite postsynaptic potentials, which are mutually cross-regulated by presynaptic autoreceptors. The GABAergic component alone effectively suppresses hippocampal sharp wave-ripples and epileptiform activity. This differentially regulated co-transmission suggests a hitherto unrecognised level of control over cortical states.

New paper on “Differential Excitatory Control of Two Parallel Basket Cell Networks in Amygdala Microcircuits” published in Plos Biology by KOKI researchers

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Researchers in the IEM HAS have discovered that in a hub of the fear circuits there are two parallel inhibitory microcircuits providing an equally strong control on neuronal spiking. These two inhibitory networks, however, receive distinct excitatory inputs. For instance, the AMPA receptor content in excitatory synapses along the dendrites of interneurons that belong to the two inhibitory circuits is different revealed by STORM. This study, in which neuroanatomical and electrophysiological techniques were combined with optogenetics, uncovered organizing principles of wiring between principal cells and inhibitory interneurons in a fear circuit that may be a general feature in cortical networks.

Analogue modulation of back-propagating action potentials enables dendritic hybrid signalling

New paper on “Analogue modulation of back-propagating action potentials enables dendritic hybrid signalling” published in Nature Communications by KOKI Scientists

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Researcher from the IEM-HAS discovered that back-propagating action potentials are not simply digital feedback signals in dendrites but also carry analogue information about the overall state of neurons. Using dendritic recordings, calcium imaging and glutamate-uncaging methods they showed that the analogue information about the somatic membrane potential modulate the dendritic action potential waveforms and local calcium influx enabling analog state-dependent long-term synaptic plasticity.

Dual-channel 3D-STORM image of an axon terminal decorated with CB1 cannabinoid receptors

Anatomical evidence for protein translation in nerve endings with STORM

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Electron microscopic studies failed to detect the translation machinery in mature mammalian axon terminals for decades. In this study, exploiting the single-molecule sensitivity of N-STORM, scientists from KOKI and the Albert Einstein College of Medicine demonstrated that ribosomal RNA and protein are present in inhibitory axon terminals of the hippocampus, and that local presynaptic protein synthesis is required for the long-term plasticity at these synapses.

N-STORM image

New paper on “Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke” published in Nature Communications by hungarian scientists

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“We show that selective elimination of microglia results in dysregulated neuronal activity and increased brain injury after experimental stroke in mice. Microglia-neuron interactions were visualized by N-STORM super-resolution microscopy, which revealed the formation of a direct contact between microglial processes and the neuronal cell body and identified clusters of microglial P2Y12 receptors at microglia-neuron contact sites.

Közös ügyünk a kutatók sikere

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A Nikon mikroszkópok hazai képviselőjeként kap­csolódott be a Nemzeti Agykutatási Programba (NAP) az Auro-Science Consulting Kft. Soós Miklós ügyvezető igazgatót és Kun Andrást, a cég Képalkotás üzletágának vezetőjét arról kérdeztük, milyen hatással lehet a tudományra egy ilyen együttműködés.

New Paper on „Correlated confocal and super-resolution imaging by VividSTORM” published in Nature Protocols by KOKI Scientists

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Abstract: Single-molecule localization microscopy (SMLM) is rapidly gaining popularity in the life sciences as an efficient approach to visualize molecular distribution with nanoscale precision. However, it has been challenging to obtain and analyze such data within a cellular context in tissue preparations. Here we describe a 5-d tissue processing and immunostaining procedure that is optimized for SMLM, and we provide …