After electrophysiological recording, the biocityn filled hippocampal interneuron (PV+ Basket Cell, in mouse brain slice) was visualised by using two different streptavidin conjugated Alexa-dyes together by accident (Streptavidin-Alexa488 and Straptavidin-Alexa594).
The photo was taken with A1R (with 60x magnification). In the deeper layers, the Alexa-594 had weaker labeling, which caused a beautiful color gradient from red to green in the axon and dendrites of the cell.
Welcome to our galaxy.(The spring basket edited in Photoshop)
These cell can form entire galaxies…:)