STORM image

New paper in Acta Neuropathologica by KOKI researchers, using correlated STORM super-resolution imaging: “Microglia control the spread of neurotropic virus infection via P2Y12 signalling and recruit monocytes through P2Y12-independent mechanisms”

Auro Science News

“Using a well-established model of alphaherpesvirus infection that reaches the brain exclusively via retrograde transsynaptic spread from the periphery, and in vivo two-photon imaging combined with high resolution microscopy, we show that microglia are recruited to and isolate infected neurons within hours. Selective elimination of microglia results in a marked increase in the spread of infection and egress of viral particles into the brain parenchyma, which are associated with diverse neurological symptoms. Microglia recruitment and clearance of infected cells require cell-autonomous P2Y12 signalling in microglia, triggered by nucleotides released from affected neurons. Microglia also contribute to monocyte recruitment into the inflamed brain. These mechanisms could be important for the early recongition and elimination of injured neurons by microglia, and for the regulation of inflammatory processes by microglia in the brain.”

Image: “STORM superresolution microscopy reveals P2Y12 receptors clustering at microglial cell membranes contacting virus-infected neurons. Neurons are identified by GFP expression with immediate-early kinetics indicating early stages of neurotropic virus (PRV) infection.”

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